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Application of capillary electrophoresis with different sample stacking strategies for the determination of a group of nonsteroidal anti‐inflammatory drugs in the low μg ∵ L −1 concentration range
Author(s) -
Macià Alba,
Borrull Francesc,
Aguilar Carme,
Calull Marta
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305707
Subject(s) - capillary electrophoresis , chromatography , stacking , chemistry , electrolyte , analytical chemistry (journal) , solvent , solid phase extraction , capillary action , extraction (chemistry) , methanol , materials science , electrode , organic chemistry , composite material
Several on‐column sample preconcentration modes – large‐volume sample stacking using the EOF pump (LVSEP), LVSEP with anion‐selective exhaustive injection (LVSEP‐ASEI) and field‐amplified sample injection with sample matrix removal using the electroosmotic flow (EOF) pump (FAEP) – were used to analyze some nonsteroidal anti‐inflammatory drugs (NSAIDs) by capillary electrophoresis, and then compared. Methanol was the background electrolyte solvent to suppress the EOF. The effect of the type and length of the solvent plug, and the sample injection time were investigated in FAEP to determine the conditions that provided the best response. LVSEP, LVSEP‐ASEI, and FAEP improved the sensitivity of the peak area by 100‐, 1200‐, and 1800‐fold, respectively. The methodology developed, in combination with solid‐phase extraction (SPE), was applied to the analysis of water samples.