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Capillary electrochromatographic analysis of barbiturates in serum
Author(s) -
Ohyama Kaname,
Wada Mitsuhiro,
Lord Gwyn A.,
Ohba Yoshihito,
Fujishita Osamu,
Nakashima Kenichiro,
Lim Chang Kee,
Kuroda Naotaka
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305703
Subject(s) - barbital , secobarbital , chromatography , capillary electrochromatography , chemistry , capillary action , repeatability , hexobarbital , phenobarbital , capillary electrophoresis , materials science , anesthesia , pentobarbital , pharmacology , biochemistry , in vitro , composite material , medicine , microsome
A capillary electrochromatographic method was developed for the separation of barbiturates. The separation was optimized in a 75 μm ID capillary, packed with 3‐(1,8‐naphthalimido)propyl‐modified silyl silica gel (NAIP), studying the effect of buffer pH, buffer concentration, and mobile phase composition. Using an applied voltage of 20 kV and the short‐end injection method (9 cm capillary effective length), the mobile phase of 1.0 m M citrate buffer (pH 5.0) containing 40% methanol provided the baseline separation of barbital, phenobarbital, secobarbital, and thiopental (internal standard) in less than 4.5 min. The method was successfully applied to the analysis of barbiturates in human serum. Under the optimal conditions, good repeatability and linearity were obtained in the range of 2.90–43.29 μg/mL for barbital, phenobarbital, and secobarbital.