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Indirect resolution of ß‐blocker agents by reversed‐phase capillary electrochromatography
Author(s) -
Aturki Zeineb,
Vichi Francesca,
Messina Antonella,
Sinibaldi Massimo
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305691
Subject(s) - capillary electrochromatography , chemistry , atenolol , chromatography , enantiomer , electrochromatography , metoprolol , oxprenolol , pindolol , resolution (logic) , detection limit , oxazepam , derivatization , capillary electrophoresis , propranolol , high performance liquid chromatography , benzodiazepine , stereochemistry , medicine , biochemistry , receptor , artificial intelligence , computer science , blood pressure , cardiology , radiology
The indirect resolution of five β‐adrenoreceptor blocking agents (propranolol, oxprenolol, pindolol, metoprolol, and atenolol) using precolumn derivatization with (+)‐1‐(9‐fluorenyl)ethyl chloroformate (FLEC), and capillary electrochromatography (CEC) is reported. Three octadecylsilanized (ODS) silica gel‐based stationary phases, differing in particle diameter and carbon surface density for suitability of determination of the enantiomeric composition of these substances in drugs and biological fluids, were studied. Under optimum separation conditions, all the investigated compounds were baseline‐resolved within acceptable analysis times ( i.e. , between 10–16 min). The resolution values ranged between 1.80 and 1.14, and the separation factors were in no case less than 1.07. The possible use of the developed CEC method for the determination of enantiomeric composition of β‐adrenoreceptor blocking agents in drug substances was investigated. A study, in which the enantiomers of metoprolol were examined regarding specificity, reproducibility, limit of detection, and ruggedness, was developed in accordance with some analytical procedures for method validation.