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Creatinine determination in serum by capillary electrophoresis
Author(s) -
Paroni Rita,
Fermo Isabella,
Cighetti Giuliana,
Ferrero Carlo Alberto,
Carobene Anna,
Ceriotti Ferruccio
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305687
Subject(s) - capillary electrophoresis , chromatography , creatinine , chemistry , biochemistry
Creatinine in human serum was separated in a fused‐silica capillary with H 3 PO 4 (75 mmol/L, pH 2.5) as BGE, followed by UV detection at 200 nm. Serum with methylimidazole added as internal standard was deproteinized with acetonitrile and the supernatant, after dilution with water was injected at pressure mode. Creatinine and methylimidazole were baseline‐resolved in 6.5 min. Linearity in the 0–880 μmol/L range gave an r 2 ≥ 0.998, recovery was 102 ± 2.8% ( n = 6). Enzymatic breakdown with creatininase confirmed that serum does not interfere. The within‐day and between‐days coefficient of variation (CV) were ≤ 2.16 and 2.7%, respectively. The accuracy, determined for lyophilized samples by isotope dilution gas chromatography‐mass spectrometry was ≤ ± 2.0%. The results were compared with HPLC for 32 lyophilized samples and on 27 serum pools. Capillary electrophoresis, rapid and inexpensive, seems a promising alternative to high‐performance liquid chromatography (HPLC) for creatinine determination in human serum.