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Sodium dodecyl sulfate‐capillary gel electrophoresis of polyethylene glycolylated interferon alpha
Author(s) -
Na Dong H.,
Park Eun J.,
Youn Yu S.,
Moon Byung W.,
Jo Yeong W.,
Lee Sung H.,
Kim WonBae,
Sohn Yeowon,
Lee Kang C.
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305684
Subject(s) - sodium dodecyl sulfate , chemistry , polyethylene glycol , capillary electrophoresis , chromatography , gel electrophoresis , polyacrylamide gel electrophoresis , peg ratio , mass spectrometry , electrophoresis , pegylation , coomassie brilliant blue , staining , biochemistry , medicine , finance , pathology , economics , enzyme
Sodium dodecyl sulfate‐capillary gel electrophoresis (SDS‐CGE) using a hydrophilic replaceable polymer network matrix was applied to characterize the polyethylene glycol(PEG)ylated interferon alpha (PEG‐IFN). The SDS‐CGE method resulted in a clearer resolution in both the PEG‐IFN species and the native IFN species. The distribution profile of PEGylation determined by SDS‐CGE was consistent with that obtained by SDS‐polyacrylamide gel electrophoresis (PAGE) with Coomassie blue or barium iodide staining. The result was also compared using matrix‐assisted laser desorption/ionization‐time of flight‐mass spectrometry. SDS‐CGE was also useful for monitoring the PEGylation reaction to optimize the reaction conditions, such as reaction molar ratio. This study shows the potential of SDS‐CGE as a new method for characterizing the PEGylated proteins with advantages of speed, minimal sample consumption and high resolution.