Diffusion constant in gel electrophoresis at high fields | Zendy

Krawczyk Małgorzata J. | Zendy; Dulak Józef | Zendy; Paściak Paweł | Zendy; Kułakowski Krzysztof | Zendy

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Diffusion constant in gel electrophoresis at high fields

Author(s) -

Krawczyk Małgorzata J.,

Dulak Józef,

Paściak Paweł,

Kułakowski Krzysztof

Publication year - 2004

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.200305678

Subject(s) - electrophoresis , diffusion , gel electrophoresis , lambda , fick's laws of diffusion , constant (computer programming) , hindiii , slab , electric field , pulsed field gel electrophoresis , chemistry , restriction enzyme , molecule , analytical chemistry (journal) , dna , chromatography , physics , optics , thermodynamics , biochemistry , organic chemistry , quantum mechanics , programming language , geophysics , computer science , genotype , gene

We present new measurements of the diffusion constant D in standard (slab‐gel) electrophoresis of DNA at fields up to 10 V/cm. Molecules investigated are bacteriophages: T4 of length 173 kbp and λ of length 48.5 kbp cut by restriction enzyme Hin dIII. We show, that D increases with the molecule length for electric field E above 5 V/cm. The results are interpreted within the geometration model.

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