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Defining the plant disulfide proteome
Author(s) -
Lee Kyunghee,
Lee Jungwha,
Kim Youngmin,
Bae Dongwon,
Kang Kyu Young,
Yoon Sung C.,
Lim Dongbin
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305677
Subject(s) - thioredoxin , glutaredoxin , biochemistry , chemistry , proteome , protein disulfide isomerase , protein subunit , redox , enzyme , organic chemistry , gene
There is considerable interest in redox regulation and new targets for thioredoxin and glutaredoxin are now being identified. It would be of great benefit to the field to have a list of all possible candidates for redox regulation – that is all disulfide proteins in plant. We developed a simple and very powerful method for identifying proteins with disulfide bonds in vivo. In this method, free thiols in proteins are fully blocked by alkylation, following which disulfide cysteines are converted to sulfhydryl groups by reduction. Finally, proteins with sulfhydryls are isolated by thiol affinity chromatography. Our method is unique in that membrane proteins as well as water‐soluble proteins are examined for their disulfide nature. By applying this method to Arabidopsis thaliana we identified 65 putative disulfide proteins, including 20 that had not previously been demonstrated to be regulated by redox state. The newly identified, possibly redox‐regulated proteins include: violaxanthin de‐epoxidase, two oxygen‐evolving enhancer proteins, carbonic anhydrase, photosystem I reaction center subunit N, photosystem I subunit III, S ‐adenosyl‐ L ‐methionine carboxyl methyltransferase, guanylate kinase, and bacterial mutT homolog. Possible functions of disulfide bonding in these proteins are discussed.