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Analytical potential of enzyme‐coated capillary reactors in capillary zone electrophoresis
Author(s) -
Simonet Bartolomé M.,
Ríos Angel,
Valcárcel Miguel
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305653
Subject(s) - capillary electrophoresis , chromatography , chemistry , capillary action , analyte , acetaldehyde , electrophoresis , immobilized enzyme , resolution (logic) , analytical chemistry (journal) , ethanol , enzyme , biochemistry , materials science , artificial intelligence , computer science , composite material
Abstract Enzymes immobilized on the inner surface of an electrophoretic capillary were used to increase sensitivity and resolution in capillary zone electrophoresis (CZE). Sensitivity is enhanced by inserting a piece of capillary containing the immobilized enzyme into the main capillary, located before the detector, in order to transform the analyte into a product with a higher absorptivity. This approach was used to determine ethanol. In order to improve resolution, capillary pieces containing immobilized enzymes were inserted at various strategic positions along the electrophoretic capillary. On reaching the enzyme, the analyte was converted into a product with a high electrophoretic mobility, the migration time for which was a function of the position of the enzyme reactor. This approach was applied to the separation and determination of acetaldehyde and pyruvate. Finally, the proposed method was validated with the determination of ethanol, acetaldehyde, and pyruvate in beer and wine samples.