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Chiral recognition of binaphthyl derivatives using electrokinetic chromatography and steady‐state fluorescence anisotropy: Effect of temperature
Author(s) -
Billiot Fereshteh Haddadian,
McCarroll Matthew C.,
Billiot Eugene J.,
Warner Isiah M.
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305649
Subject(s) - enantiomer , fluorescence anisotropy , anisotropy , electrokinetic phenomena , chemistry , micellar electrokinetic chromatography , fluorescence , phase (matter) , chromatography , steady state (chemistry) , fluorescence spectroscopy , analytical chemistry (journal) , stereochemistry , capillary electrophoresis , organic chemistry , biochemistry , physics , quantum mechanics , membrane
The effect of temperature on the chiral recognition of binaphthyl derivatives in the presence of poly sodium N ‐undecanoyl‐ LL ‐leucyl‐leucinate (poly LL ‐SULL) is examined using electrokinetic chromatography (EKC) and steady‐state fluorescence anisotropy. An examination of the effect of temperature suggests that the chiral recognition of 1,1'‐binaphthyl‐2,2'‐diol enantiomers improves with increasing temperature, whereas lower temperatures resulted in better enantiosolectivity in the case of 1,1'‐binaphthyl‐2,2'‐diyl hydrogen phosphate enantiomers. In addition, steady‐state fluorescence anisotropy results show that the anisotropy of the two enantiomers are different when complexed to poly‐( LL ) SULL. As would be expected, the enantiomer that binds stronger to the chiral pseudostationary phase, as evidenced by EKC experiments, had higher anisotropy values. The results of this study suggest that steady‐state fluorescence anisotropy can be used to gain further insight into chiral recognition.