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Microemulsion electrokinetic chromatography with laser‐induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine
Author(s) -
Zhang Jiyou,
Xie Jianping,
Liu Jiaqin,
Tian Jianniao,
Chen Xingguo,
Hu Zhide
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305643
Subject(s) - pseudoephedrine , chromatography , chemistry , ephedrine , microemulsion , detection limit , micellar electrokinetic chromatography , sodium dodecyl sulfate , electrokinetic phenomena , linear range , acetonitrile , laser induced fluorescence , fluorescence , pulmonary surfactant , biochemistry , physics , quantum mechanics , neuroscience , biology
A selective and sensitive microemulsion electrokinetic chromatography with laser‐induced fluorescence detection method was developed for the quantification of ephedrine (E) and pseudoephedrine (PE) derivatized with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1, 3‐diazol. By a series of optimization, a running buffer composed of 20 m M borate + microemulsion (23.3 m M Sodium dodecyl sulfate/180.85 m M 1‐butanol/16.4 m M n ‐heptane) +8% acetonitrile was applied for the separation of the derivatives. A linear relationship for E and PE was obtained in the range of 0.058–11.58 μg·mL −1 (correlation coefficient: 0.9993 for E, 0.9995 for PE), and the detection limits for E and PE were 5.3 and 3.9 ng·mL −1 . The method was applied to the analysis of the two alkaloids in Chinese traditional herbal preparations with recoveries in the range of 96.9–105.4%.