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Analysis of cyanobacterial toxins (anatoxin‐a, cylindrospermopsin, microcystin‐LR) by capillary electrophoresis
Author(s) -
Vasas Gábor,
Gáspár Attila,
Páger Csilla,
Surányi Gyula,
Máthé Csaba,
Hamvas Márta M.,
Borbely George
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305641
Subject(s) - cylindrospermopsin , micellar electrokinetic chromatography , capillary electrophoresis , chromatography , chemistry , detection limit , microcystin , electrophoresis , cyanobacteria , biology , genetics , bacteria
Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) were applied to the simultaneous separation of cyanobacterial toxins (anatoxin‐a, microcystin‐LR, cylindrospermopsin). The analytical performance data of both methods, optimized for the three toxins, were similar with a precision of migration times smaller than 0.8 RSD% and a detection limit in the range of 1–4 μg/mL, using spectrophotometric detection at 230 nm. Both methods were applied to an analysis of cyanotoxins in water bloom samples and crude cyanobacterial extracts. The results obtained indicate that, for complex matrices, the sequential application of CZE and MEKC is necessary. It is recommended to use both CE techniques for the analysis of the same sample in order to confirm the results by an orthogonal approach.