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Microdevice for separation and quantitative fraction collection
Author(s) -
Spěšný Michal,
Foret František
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305628
Subject(s) - fraction (chemistry) , chromatography , separation (statistics) , microfluidics , centrifugal force , polycarbonate , column (typography) , channel (broadcasting) , materials science , analytical chemistry (journal) , chemistry , nanotechnology , computer science , composite material , physics , mechanics , flow (mathematics) , telecommunications , computer network , frame (networking) , machine learning
A new microfluidic concept for quantitative whole‐column fraction collection of electrophoretically separated zones was developed. The prototype device, fabricated on a polycarbonate disk by injection molding, integrated electrophoretic separation channels with fraction collection reservoirs distributed along the separation channel. The microdevice was designed in a CD‐like format to use the centrifugal force for moving the liquid in the microchannels. A serpentine shape of the separation channel was selected to create segments for quantitative whole‐column fraction collection. The operation was tested with visual monitoring of isotachophoretic separation and collection of cationic dyes.