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High‐speed electrophoretic analysis of 1‐phenyl‐3‐methyl‐5‐pyrazolone derivatives of monosaccharides on a quartz microchip with whole‐channel UV detection
Author(s) -
Suzuki Shigeo,
Ishida Yoshiro,
Arai Akihiro,
Nakanishi Hiroaki,
Honda Susumu
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305626
Subject(s) - capillary electrophoresis , chromatography , electrophoresis , monosaccharide , chemistry , pyrazolone , analytical chemistry (journal) , quartz , organic chemistry , materials science , composite material
1‐Phenyl‐3‐methyl‐5‐pyrazolone (PMP) derivatives of monosaccharides were analyzed by electrophoresis on a quartz microchip with whole‐channel UV detection. Rapid separation of PMP derivatives of aldopentoses was achieved by plain‐zone electrophoresis in a neutral phosphate buffer with the height equivalent to a theoretical plate at the micrometer level. Zone electrophoresis as borate complexes was also successful for the separation of PMP derivatives of a few aldoses, which were separated within 1 min. Separation by microchip electrophoresis was compared to that by capillary electrophoresis, and the difference was discussed in terms of column efficiency and sample column capacity.