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Partial filling technique in capillary electrophoresis for the separation of phenolic isomers with sulfonatocalix[4]arene as a selector
Author(s) -
Xu Hui,
Yu XiaoDong,
Chen HongYuan
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305602
Subject(s) - calixarene , capillary electrophoresis , chemistry , chromatography , structural isomer , analytical chemistry (journal) , capillary action , absorption (acoustics) , electrophoresis , molecule , materials science , stereochemistry , organic chemistry , composite material
p ‐Sulfonatocalix[4]arene was used as a selector in capillary electrophoresis to separate phenolic positional isomers. To avoid the detection interference caused by the high UV absorption of calixarene, the partial filling technique was applied. The operation variables, including buffer, separation voltage, the concentration of the selector and the plug length of the selector zone, were systematically optimized. The detection limits of mass were in the range of 0.07–0.28 pg. Molecular modeling was used to explain the interaction between calixarene and phenolic isomers.