Premium
Direct immunoassay of estrone by capillary electrophoresis with laser‐induced fluorescence detection
Author(s) -
Su Ping,
Zhang XinXiang,
Chang WenBao
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305599
Subject(s) - immunoassay , capillary electrophoresis , estrone , chromatography , chemistry , chemiluminescence , reproducibility , fluorescence , electrophoresis , fluorescein , laser induced fluorescence , monoclonal antibody , analytical chemistry (journal) , antibody , medicine , biochemistry , optics , immunology , physics , hormone
An immunoassay for estrone (E 1 ) in women's serum, based on the competitive reaction between fluorescein‐labeled complete antigen and E 1 with limited amount of anti‐estrone monoclonal antibody is described. A thermally reversible hydrogel, poly‐ N ‐isopropylacrylamide (pNIPA), was added to the buffer to improve the reproducibility. With a laser‐induced fluorescence (LIF) detector, the capillary electrophoretic immunoassay (CEIA) can be applied to determine E 1 at a concentration lower than 19.6 pg/mL. The E 1 levels in ten normal women's serum were measured at the range of 118.6–222.0 pg/mL.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom