Separation and determination synchronously by multichannel mode‐filtered light capillary electrochromatography | Zendy

Zuo Xinbing | Zendy; Wang Kemin | Zendy; Zhou Leiji | Zendy; Huang Shasheng | Zendy

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Separation and determination synchronously by multichannel mode‐filtered light capillary electrochromatography

Author(s) -

Zuo Xinbing,

Wang Kemin,

Zhou Leiji,

Huang Shasheng

Publication year - 2003

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.200305596

Subject(s) - capillary electrochromatography , capillary action , capillary electrophoresis , electrochromatography , chromatography , analytical chemistry (journal) , bovine serum albumin , chemistry , coating , phase (matter) , fourier transform infrared spectroscopy , materials science , optics , nanotechnology , composite material , physics , organic chemistry

Mode‐filtered light capillary electrophoresis (CE) was developed for capillary electrochromatography (CEC) by applying annular columns of different size (250, 320, and 530 μm) and a bovine serum albumin (BSA)‐modified chiral stationary phase. BSA was encapsulated on the pretreated surface of an optic fiber and the inner wall of a capillary using sol‐gel technique with a resultant larger active stationary phase area. The coating was examined by atomic force microscopy (AFM) and Fourier transform infrared (FT‐IR). The separation of DL ‐tryptophan (10 m M ) was investigated under different voltage/current conditions.

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