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Cross‐linked coatings for electrophoretic separations in poly(dimethylsiloxane) microchannels
Author(s) -
Hu Shuwen,
Ren Xueqin,
Bachman Mark,
Sims Christopher E.,
Li G. P.,
Allbritton Nancy
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305592
Subject(s) - monomer , fluorescein , electrophoresis , chromatography , analyte , chemistry , polymerization , materials science , chemical engineering , analytical chemistry (journal) , fluorescence , polymer , organic chemistry , optics , physics , engineering
We have developed a strategy using ultraviolet light to polymerize mixed monomer solutions onto the surface of a poly(dimethylsiloxane) (PDMS) microdevice. By including monomers with different chemical properties, electrophoretic separations were optimized for a test set of analytes. The properties of surfaces grafted with a single neutral monomer, a neutral and a negative monomer, or a neutral, negative, and cross‐linking monomer were assessed. The highest quality separations were achieved in channels with cross‐linked coatings. The separation efficiency for biologically relevant peptides (kinase substrates) on these surfaces was as high as 18 600 theoretical plates in a 2.5 cm channel. The test peptides were fluorescein‐AEEEIYGEFEAKKKK, fluorescein‐GRPRAATFAEG, fluorescein‐GRPRAA(T‐PO 3 )FAEG, fluorescein‐DLDVPIP GRFDRRVSVAAE, and fluorescein‐DLDVPIPGRFDRRV(S‐PO 3 )VAAE. Separations between two different peptides occurred in as little as 400 ms after injection into the separation channel. The simultaneous separation of five kinase and phosphatase substrates was also demonstrated. By carefully selecting mixtures of monomers with the appropriate properties, it may be possible to tailor the surface of PDMS for a large number of different electrophoretic separations.