Premium
Free solution mobility of small single‐stranded oligonucleotides with variable charge densities
Author(s) -
Dong Qian,
Stellwagen Earle,
Dagle John M.,
Stellwagen Nancy C.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305589
Subject(s) - phosphoramidate , chemistry , capillary electrophoresis , diffusion , electrophoresis , oligonucleotide , analytical chemistry (journal) , phosphate , chemical physics , logarithm , crystallography , dna , chromatography , stereochemistry , thermodynamics , organic chemistry , physics , biochemistry , mathematical analysis , mathematics
The free solution mobilities of six single‐stranded 16‐nucleotide DNA oligomers with the same sequence, containing up to 11 neutral phosphoramidate internucleoside linkages, have been measured by capillary electrophoresis. The mobilities of the partially charged oligomers increase linearly with the logarithm of increasing charge density, as predicted by the Manning theory of electrophoresis (G. S. Manning, J. Phys. Chem . 1981, 85 , 1506–1515). For comparison, the mobilities of eight fully charged single‐stranded oligomers containing similar numbers of charged phosphate residues have also been measured. The mobilities of the variable length, fully charged oligomers increase more rapidly with the increasing number of charged phosphate residues than the mobilities of the constant size, partially charged phosphoramidate derivatives, because of the larger diffusion coefficients of the modified oligomers.