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Capillary electrochromatography and preconcentration of neutral compounds on poly(dimethylsiloxane) microchips
Author(s) -
Ro Kyung Won,
Chang WooJin,
Kim Ho,
Koo YoonMo,
Hahn Jong Hoon
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305568
Subject(s) - capillary electrochromatography , frit , electrochromatography , elution , chromatography , capillary action , bead , materials science , acetonitrile , analyte , chemistry , analytical chemistry (journal) , capillary electrophoresis , composite material , metallurgy
Capillary electrochromatography (CEC) and preconcentration of neutral compounds have been realized on poly(dimethylsiloxane) (PDMS) microchips. The channels are coated with polyelectrolyte multilayers to avoid absorption of hydrophobic analytes into PDMS. The structures of a microchip include an injector and a bead chamber with integrated frits, where the particles of the stationary phase are completely retained. Dimensions of the frit structures are 25 μm×20 μm, and the space between the structures is 3 μm. A neutral compound, BODIPY, that is strongly absorbed into native PDMS, is successfully and selectively retained on octadecylsilane‐coated silica beads in the bead chamber with a concentration enhancement of up to 100 times and eluted with elution buffer solution containing 70% acetonitrile. Preconcentrations and CEC separations of coumarins have been conducted with the same device and achieved complete separations in less than 50 s.