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Validation of capillary electrophoresis – mass spectrometry methods for the analysis of a pharmaceutical formulation
Author(s) -
Geiser Laurent,
Rudaz Serge,
Veuthey JeanLuc
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305564
Subject(s) - capillary electrophoresis , mass spectrometry , chromatography , capillary electrophoresis–mass spectrometry , chemistry , electrospray ionization , capillary action , analytical chemistry (journal) , electrospray , resolution (logic) , quantitative analysis (chemistry) , materials science , computer science , artificial intelligence , composite material
Abstract The coupling of capillary electrophoresis (CE) to mass spectrometry (MS) with an electrospray ionization (ESI) technique is generally performed for qualitative applications, and only few quantitative results have been reported. This paper investigates the validation of a CE‐ESI‐MS method for the analysis of a pharmaceutical formulation containing lidocaine. Some important ESI criteria are discussed including sheath‐liquid composition, nebulizing gas pressure and position of the CE capillary outlet. After optimization of these parameters, an intermediate precision of about 5% was achieved. The latter, as well as efficiency and resolution, were compared to those achieved with UV detection. Besides, a multiple injection procedure was developed to reduce analysis time per sample and was successfully applied to both UV and MS detectors. The validation results achieved by multiple injections were identical to those obtained with classical injection, but afforded a gain of time by a factor of 2.5.