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Separation of capsular polysaccharide K4 and defructosylated K4 by high‐performance capillary electrophoresis
Author(s) -
Volpi Nicola
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305563
Subject(s) - capillary electrophoresis , chromatography , chemistry , sodium dodecyl sulfate , polysaccharide , electrokinetic phenomena , electrophoresis , biochemistry
Abstract A rapid, highly sensitive and reproducible high‐performance capillary electrophoresis (HPCE) method (electrokinetic chromatography with sodium dodecyl sulfate) is described for the determination of the polysaccharide from the uropathogenic Escherichia coli K4 bacteria (05:K4:H4) and its defructosylated product. The two polyanions, K4 and defructosylated K4, are separated and readily determined within 30 min on an uncoated fused‐silica capillary using normal polarity at 20 kV and detection at 200 nm. A linear relationship was found for the two polysaccharides over a wide range of concentrations, from approximately 30 ng (0.5 μg/μL) to 210 ng (3.5 μg/μL). The described method was used to evaluate the defructosylation process of K4 under drastic acid conditions.