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Capillary electrophoresis as a simple and sensitive method to study polysaccharides of Sinorhizobium sp. NGR234
Author(s) -
Fraysse Nicolas,
Verollet Christel,
Couderc François,
Poinsot Véréna
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305555
Subject(s) - capillary electrophoresis , monosaccharide , polysaccharide , chromatography , chemistry , hydrolysis , electrophoresis , mass spectrometry , biochemistry
We report the minimum amounts of polysaccharides (6.5 ng) that can be studied using capillary electrophoresis‐laser‐induced flurescence (CE‐LIF) and describe the separation of the various monosaccharides susceptible to occur in Sinorhizobium cell wall. On‐gel hydrolysis of the polysaccharides is described. It allowed the easy and rapid determination of the monosaccharide composition of the excised lipopolysaccharides. The composition obtained fits with gas chromatography‐mass spectrometry (GC‐MS) results. This study underlines the simplicity of using CE‐LIF, which remains an entirely “water medium” method, unlike GC‐MS which is, in addition, less sensitive (at least 100‐fold) than CE‐LIF.