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Enantioseparation of dihydrofurocoumarin derivatives by various separation modes of capillary electrophoresis
Author(s) -
Egger Matthew D.,
Liu Ying,
Sevčík Juraj,
Tesařová Eva,
Rozhkov Roman,
Larock Richard C.,
Armstrong Daniel W.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305511
Subject(s) - capillary electrophoresis , cyclodextrin , chemistry , chromatography , electrophoresis , sulfation , polarity (international relations) , combinatorial chemistry , beta cyclodextrins , biochemistry , cell
Chiral dihydrofurocoumarin compounds are currently the focus of industrial and pharmacological research. These derivatives have been shown to possess many physiological properties that could be medically beneficial. This work proposes four different chiral separation methods using capillary electrophoresis and micellar capillary electrophoresis (MCE). Several different cyclodextrin chiral selectors were examined to evaluate their effectiveness in the enantioseparation of dihydrofurocoumarins. In addition, the effects of the chiral selector concentration, the presence of an organic modifier, run buffer pH, and in two cases, the ratio between the chiral selector and an additional charged pseudophase were investigated. Overall, the best separations for this class of chiral compounds were achieved using sulfated β‐cyclodextrins at low pH in the reversed polarity mode.