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Violet light emitting diode‐induced fluorescence detection combined with on‐line sample concentration techniques for use in capillary electrophoresis
Author(s) -
Tsai ChihHsin,
Huang HsuanMing,
Lin ChengHuang
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305505
Subject(s) - micellar electrokinetic chromatography , capillary electrophoresis , detection limit , chemistry , chromatography , fluorescence , analytical chemistry (journal) , capillary action , fluorescence spectroscopy , electrophoresis , laser induced fluorescence , reserpine , materials science , optics , medicine , physics , composite material , endocrinology
The first application of a violet light‐emitting diode (LED) for fluorescence detection in capillary electrophoresis (CE) is described. The utility of violet LED (peak emission wavelength at 410 nm, ∼ 2 mW) for fluorescence detection is demonstrated by examining reserpine and dopamine‐labeled NDA (naphthalene‐2,3‐dicarboxaldehyde), respectively. The detection limit for reserpine was determined to be 2.5×10 −6 M by normal micellar electrokinetic capillary chromatography (MEKC) and this was improved to 2.0×10 −9 M and 2.0×10 −10 M when sweeping‐MEKC and cation‐selective exhaustive injection (CSEI)‐sweep‐MEKC techniques were applied, respectively. In addition, the detection limit of NDA‐labeled dopamine was determined to be 6.3×10 −6 M by means of normal MEKC and this was improved to 3.0×10 −8 M when the sweeping‐MEKC mode was applied.