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Evaluation of teicoplanin chiral stationary phases of 3.5 and 5 μm inside diameter silica microparticles by polar‐organic mode capillary electrochromatography
Author(s) -
Catarcini Paolo,
Fanali Salvatore,
Presutti Carla,
D'Acquarica Ilaria,
Gasparrini Francesco
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305504
Subject(s) - capillary electrochromatography , chemistry , chromatography , oxprenolol , alprenolol , pindolol , teicoplanin , capillary action , phase (matter) , resolution (logic) , enantiomer , analytical chemistry (journal) , materials science , stereochemistry , capillary electrophoresis , propranolol , organic chemistry , artificial intelligence , bacteria , receptor , computer science , composite material , biology , genetics , staphylococcus aureus , biochemistry , vancomycin , medicine
Different types of fused‐silica capillaries of 75 μm inside diameter (ID) were packed, namely type A and B, and evaluated for the direct resolution of racemates of several basic compounds by enantioselective capillary electrochromatography (e‐CEC). Type A was packed with a chiral stationary phase (CSP) containing teicoplanin (TE) mixed with silica microparticles (3:1 w/w) while type B contained only the TE‐CSP. In both cases, particles of different sizes (3.5 and 5 μm ID) were employed. A polar‐organic mobile phase containing methanol‐acetonitrile (60–40% v/v and 0.05% w/v ammonium acetate was used. Several β‐blockers (alprenolol, oxprenolol, metoprolol, pindolol, salbutamol, propranolol, atenolol, acebutolol) were baseline‐enantioresolved with both capillary types, in very short times.