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Determination of L ‐hyoscyamine in atropine and D ‐hyoscyamine in L ‐hyoscyamine by chiral capillary electrophoresis as an alternative to polarimetry
Author(s) -
Heine Stephanie,
Ebert Kirstin,
Blaschke Gottfried
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305492
Subject(s) - hyoscyamine , chromatography , capillary electrophoresis , chemistry , biochemistry , solanaceae , gene
A method for the separation of atropine enantiomers, D ‐ and L ‐hyoscyamine by capillary electrophoresis (CE) has been developed and validated. The advantages of the CE method compared with polarimetry include smaller amounts of analytes and a lower limit of detection of the unwanted enantiomer. Moreover, the present method enables a baseline separation of the analytes and tropic acid, one of the typical impurities of atropine. The developed enantioseparation of atropine was performed using a commercially available sulfated β‐cyclodextrin and was validated for the determination of L ‐hyoscyamine in atropine as well as for the enantiomeric purity of L ‐hyoscyamine.