Determination of L ‐hyoscyamine in atropine and D ‐hyoscyamine in L ‐hyoscyamine by chiral capillary electrophoresis as an alternative to polarimetry

A method for the separation of atropine enantiomers, D ‐ and L ‐hyoscyamine by capillary electrophoresis (CE) has been developed and validated. The advantages of the CE method compared with polarimetry include smaller amounts of analytes and a lower limit of detection of the unwanted enantiomer. Moreover, the present method enables a baseline separation of the analytes and tropic acid, one of the typical impurities of atropine. The developed enantioseparation of atropine was performed using a commercially available sulfated β‐cyclodextrin and was validated for the determination of L ‐hyoscyamine in atropine as well as for the enantiomeric purity of L ‐hyoscyamine.