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Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer
Author(s) -
Chen Xiaoming,
Jin Wenhai,
Qin Feng,
Liu Yueqi,
Zou Hanfa,
Guo Baochuan
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305484
Subject(s) - enantiomer , cellulose , capillary electrochromatography , capillary action , chromatography , chemistry , derivative (finance) , capillary electrophoresis , materials science , organic chemistry , composite material , financial economics , economics
Positively charged chiral stationary phases (CSPs) were prepared for capillary electrochromatography (CEC) separation of enantiomers by chemically immobilizing cellulose derivatives onto diethylenetriaminopropylated silica (DEAPS) with tolylene‐2,4‐diisocyanate (TDI) as a spacer reagent. Anodic electroosmotic mobility was observed in both nonaqueous and aqueous mobile phases due to the positively charged amines on the surface of the prepared CSPs. For comparison, the traditionally used 3‐aminopropyl silica (APS) was also adopted as the base material instead of DEAPS to prepare CSP. It was observed that the EOF on the DEAPS‐based CSP was 18%–60% higher than that on the APS‐based CSP under nonaqueous mobile phase conditions. Separation of enantiomers in CEC was performed on the positively charged CSPs with the nonaqueous mobile phases of pure ethanol or mixture of hexane‐alcohol and the aqueous phases of acetonitrile‐water or 95% ethanol. Fast separation of enantiomers was achieved on the newly prepared CSPs.