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Chiral resolution of diastereomeric di‐ and tripeptides on a teicoplanin aglycone phase by capillary electrochromatography
Author(s) -
Schmid Martin G.,
Grobuschek Nina,
Pessenhofer Verena,
Klostius Andrea,
Gübitz Gerald
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305482
Subject(s) - tripeptide , diastereomer , capillary electrochromatography , chemistry , aglycone , phase (matter) , enantiomer , chromatography , teicoplanin , resolution (logic) , organic chemistry , stationary phase , amino acid , biochemistry , vancomycin , artificial intelligence , glycoside , biology , bacteria , computer science , genetics , staphylococcus aureus
The chiral separation ability of a capillary packed with teicoplanin aglycone as a chiral stationary phase was investigated. This stationary phase was used successfully for chiral resolution of both diastereomeric dipeptides and tripeptides possessing one or two chiral centers. The composition of the mobile phase was shown to be crucial for separation. The use of reversed‐phase mode was clearly superior to the polar‐organic mode. The nature of the organic modifier was found to have a marked influence on separation. After optimizing conditions, all diasteromeric dipeptides and tripeptides investigated were baseline‐resolved, however, it was not possible to find a uniform mobile phase showing optimal results for all peptides investigated.