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Nonconventional synthesis and characterization of ultrahigh‐molar‐mass polyacrylamides
Author(s) -
Cretich Marina,
Chelaru Carmen,
Pirri Giovanna,
Levi Marinella,
Clerici Gigliola,
Chiari Marcella
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305479
Subject(s) - polymer , molar mass , polyacrylamide , viscosity , capillary electrophoresis , intrinsic viscosity , solubility , electrophoresis , chromatography , chemistry , linearity , characterization (materials science) , dna , resolution (logic) , materials science , analytical chemistry (journal) , chemical engineering , nanotechnology , polymer chemistry , organic chemistry , computer science , physics , biochemistry , quantum mechanics , artificial intelligence , engineering , composite material
In spite of the significant progresses in the field of replaceable sieving matrices for separating DNA in capillary electrophoresis (CE), an intense research activity is still going on to improve the separation of large size DNA sequencing fragments. There are evidences, both from experimental and theoretical sides that the resolution of these fragments, at the single base, requires the use of sieving matrices comprised of long chain linear polymers. In the separation of DNA fragments by CE are of upmost importance: (i) the complete solubility of the polymer, (ii) the linearity of the chain, (iii) the achievement of ultrahigh viscosity in dilute solutions. The aim of this work is the synthesis of ultrahigh‐molecular‐weight polymers which possess the three requirements mentioned above by employing a nonconventional method. We demonstrate that the sieving performance of polyacrylamide is directly correlated to its intrinsic viscosity.