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Determination of bupivacaine and metabolites in rat urine using capillary electrophoresis with mass spectrometric detection
Author(s) -
Krisko Ryan M.,
Schieferecke Mark A.,
Williams Todd D.,
Lunte Craig E.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305469
Subject(s) - chromatography , chemistry , capillary electrophoresis , mass spectrometry , urine , solid phase extraction , capillary electrophoresis–mass spectrometry , tandem mass spectrometry , quadrupole time of flight , electrospray ionization , biochemistry
A method using capillary electrophoresis‐mass spectrometry (CE‐MS) was developed for the structural elucidation of bupivacaine and metabolites in rat urine. Prior to CE‐MS analysis, solid‐phase extraction (SPE) was used for sample cleanup and preconcentration purposes. Exact mass and tandem mass spectrometric (MS/MS) experiments were performed to obtain structural information about the unknown metabolites. Two instruments with different mass analyzers were used for mass spectrometric detection. A quadrupole time‐of‐flight (Q‐TOF) and a magnetic sector hybrid instrument were coupled to CE and used for the analysis of urine extracts. Hydroxybupivacaine as well as five other isomerically different metabolites were detected including methoxylated bupivacaine.