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Enantioseparation of warfarin and its metabolites by capillary zone electrophoresis
Author(s) -
Zhou Qingyu,
Yau WaiPing,
Chan Eli
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305441
Subject(s) - capillary electrophoresis , chromatography , chemistry , enantiomer , repeatability , electrophoresis , warfarin , resolution (logic) , selectivity , stereochemistry , biochemistry , computer science , cardiology , catalysis , atrial fibrillation , medicine , artificial intelligence
A capillary zone electrophoresis (CZE) method with direct ultraviolet (UV)‐absorbance detection is presented for the simultaneous enantiomeric separation of warfarin and its main metabolites, including warfarin alcohols, 4’‐, 6‐, and 7‐hydroxywarfarin, using highly sulfated β‐cyclodextrin (HS‐β‐CD) as the chiral selector. This chiral separation method was optimized in terms of the electrophoretic parameters, which included the concentration of HS‐β‐CD used, the type and composition of organic modifier added to the background electrolyte (BGE) buffer, and the BGE buffer pH. Chiral separation of warfarin and its major metabolites was achieved with high resolution, selectivity, efficiency, repeatability, and reproducibility. This optimized chiral analysis of warfarin along with its metabolites was completed within a satisfactory electrophoresis time of 20 min.