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Optimization of the chiral resolution of baclofen by capillary electrophoresis using β‐cyclodextrin as the chiral selector
Author(s) -
Ali Imran,
AboulEnein Hassan Y.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305439
Subject(s) - capillary electrophoresis , acetonitrile , chromatography , chemistry , cyclodextrin , resolution (logic) , electrophoresis , electrolyte , phosphate buffered saline , capillary action , chiral resolution , enantiomer , materials science , stereochemistry , electrode , artificial intelligence , computer science , composite material
The chiral resolution of baclofen was achieved by capillary electrophoresis using a fused‐silica capillary (60 cm×75 μm ID). The background electrolyte (BGE) was phosphate buffer (pH 7.0, 50 m M )‐acetonitrile (95:5 v/v) containing 10 m M β‐cyclodextrin. The applied voltage was 15 kV. The values of α and R s were 1.06 and 1.00, respectively. The electrophoretic conditions were optimized varying the pH and the ionic strength of the BGE, concentrations of β‐cyclodextrin and acetonitrile and the applied voltage.