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Flow injection‐capillary electrophoresis system with contactless conductivity detection and hydrostatic pressure generated flow. Application to the quantitative analysis of inorganic anions in water samples
Author(s) -
Kubáň Pavel,
Kubáň Petr,
Kubáň Vlastimil
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305433
Subject(s) - capillary electrophoresis , electrolyte , repeatability , chromatography , analytical chemistry (journal) , electrokinetic phenomena , chemistry , thermal conductivity detector , detection limit , conductivity , capillary action , flow injection analysis , volumetric flow rate , materials science , electrode , physics , quantum mechanics , gas chromatography , composite material
A simple and inexpensive flow injection‐capillary electrophoresis (FI‐CE) system with contactless conductivity detection (CCD) for automated quantitative analysis of chloride, nitrate, and sulfate in various water samples is demonstrated. A glass bottle containing the background electrolyte that is raised above the FI‐CE interface generates a pulse‐free, highly reproducible flow of the electrolyte through the FI‐CE interface. The system operates at a flow rate of 300 μLmin −1 with an injection volume of only 4 μL. The repeatability of peak areas ( n = 18) was better than 0.81% RSD and the sample throughput was 90 samples per hour using the background electrolyte containing 12 m M L ‐histidine adjusted to pH 4.00 with acetic acid. The limits of detection were better than 125 μgL −1 and were comparable to those obtained by conventional CE systems with CCD. Various calibration methods for FI‐CE system with electrokinetic injection were tested and their suitability for the analysis of anions in real samples was evaluated.