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A versatile electrophoresis system for the analysis of high‐ and low‐molecular‐weight proteins
Author(s) -
Tastet Christophe,
Lescuyer Pierre,
Diemer Hélène,
Luche Sylvie,
van Dorsselaer Alain,
Rabilloud Thierry
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305400
Subject(s) - electrophoresis , chemistry , chromatography , buffer (optical fiber) , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , stacking , resolution (logic) , sodium dodecyl sulfate , gel electrophoresis , acrylamide , counterion , capillary electrophoresis , tris , sodium , ion , biochemistry , enzyme , organic chemistry , monomer , telecommunications , artificial intelligence , computer science , polymer
A new, versatile, multiphasic buffer system for high‐resolution sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of proteins in the relative molecular weight range of 300 000–3000 Da is described. The system, based on the theory of multiphasic zone electrophoresis, allows complete stacking and destacking of proteins in the above M r range. The buffer system uses taurine and chloride as trailing and leading ion, respectively, and Tris, at a pH close to its p K a , as the buffering counterion. Coupled with limited variation in the acrylamide concentration, this electrophoresis system allows to tailor the resolution in the 6–200 kDa M r range, with minimal difficulties in the post electrophoretic identification processes.