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Electrophoretic analysis of quinone anion radicals in acetonitrile solutions using an on‐line radical generator
Author(s) -
Esaka Yukihiro,
Okumura Noriko,
Uno Bunji,
Goto Masashi
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305395
Subject(s) - radical , chemistry , acetonitrile , anthraquinone , quinone , photochemistry , ion , capillary electrophoresis , electrolysis , electrophoresis , inorganic chemistry , chromatography , organic chemistry , electrode , electrolyte
We have investigated analysis of anion radicals of phenanthrenequinone (PhQ) and anthraquinone (AQ) using acetonitrile‐capillary electrophoresis (CE) under anaerobic conditions. PhQ and AQ have relatively high negative reduction potentials meaning that their anion radicals are re‐oxidized quite readily by the surrounding O 2 to disappear during analysis and we failed to detect them with our previous system. In this work, we have developed an on‐line system combining a unique electrolysis cell for generation of the radicals and a CE unit to keep the analysis system free from external O 2 molecules and to reduce analysis time remarkably. As a result, electrophoretic detection of the anion radicals of PhQ and AQ has been achieved. Furthermore, we have observed hydrogen‐bonding interaction between the anion radicals and dimethylurea (DMU) using the present system and have indicated a characteristic interaction of the anion radical of PhQ as an ortho ‐quinone with DMU.