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Background‐free, fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counterion dyes, zincon and ethyl violet
Author(s) -
Choi JungKap,
Tak KeongHoon,
Jin LiTai,
Hwang SunYoung,
Kwon TaeIk,
Yoo GyurngSoo
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200290020
Subject(s) - staining , chemistry , coomassie brilliant blue , stain , counterion , chromatography , sodium dodecyl sulfate , electrophoresis , polyacrylamide gel electrophoresis , gel electrophoresis , sodium , polyacrylamide , alizarin red , biochemistry , polymer chemistry , ion , organic chemistry , biology , enzyme , genetics
A background‐free, fast protein staining method in polyacrylamide gel electrophoresis using an acidic dye, zincon (ZC) and a basic dye, ethyl violet (EV) is described. It is based on the counterion dye staining technique that employs two oppositely charged dyes to form an ion‐pair complex in staining solution. The selective binding of free dye molecules to proteins in acidic solution produces bluish violet‐colored bands. It is a rapid and end‐point staining procedure, involving only fixing and staining steps that are completed in 1–1.5 h. The detection limit of this method is 8–15 ng of protein that is comparable to the sensitivity of the colloidal Coomassie Brilliant Blue G (CBBG) stain. Due to its sensitivity and speed, this stain may be more practical than any other dye‐based stains for routine laboratory purposes.