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Peak broadening in microchip electrophoresis: A discussion of the theoretical background
Author(s) -
Gaš Bohuslav,
Kenndler Ernst
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200290003
Subject(s) - electrophoresis , chromatography , analytical chemistry (journal) , chemistry
A review on peak (band, zone) broadening in electromigration separation methods is presented, mainly covering articles published between the begining of 2000 and middle of 2002. Most attention is drawn to work dealing with microchip electrophoresis performed in micrototal analysis systems (μTAS) or the lab‐on‐a‐chip, but many of the results are significant for capillary zone electrophoresis in general. The paper reviews the theoretical background of the peak dispersion due to the geometry of the separation channel, the transversal nonhomogeneity of the electroosmootic flow, and electromigration dispersion (sample overload) connected with the occurrence of the system zones (system peaks, system eigenpeaks).