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A continuous‐flow instrument for the determination of linear polyacrylamide stability
Author(s) -
Klepárník Karel,
Mikuška Pavel
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200035921
Subject(s) - hydrolysis , chemistry , polyacrylamide , chromatography , capillary electrophoresis , amide , volumetric flow rate , electrophoresis , capillary action , analytical chemistry (journal) , biochemistry , materials science , polymer chemistry , thermodynamics , physics , composite material
Alkaline hydrolysis of high‐molecular‐mass (10 MDa) linear polyacrylamide, a widely used replaceable sieving medium for the capillary electrophoresis of DNA fragments and proteins, was investigated. The release rate of ammonia, a product of amide group hydrolysis, was monitored by a high‐sensitivity continuous‐flow system. The experimental results show a rapid onset of hydrolysis at a temperature of 70°C. While the degree of 1 h hydrolysis was evaluated to reach about 16% at 70°C and pH 13, it drops to 5% at 50°C and pH 12.5.