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Nonaqueous capillary electrophoretic separation and thermo‐optical absorbance detection of five tricyclic antidepressants and metabolism of amitriptyline by Cunninghamella elegans
Author(s) -
Li XingFang,
Liu ChungSheng,
Roos Pieter,
Hansen Eugene B.,
Cerniglia Carl E.,
Dovichi Norman J.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191821
Subject(s) - capillary electrophoresis , chromatography , absorbance , chemistry , amitriptyline , ammonium acetate , electrophoresis , high performance liquid chromatography , pharmacology , medicine
We developed a technique based on nonaqueous capillary electrophoresis and laser‐based thermo‐optical absorbance detection to assay five antidepressants with similar structures and mass‐to‐charge ratios. A mixture of methanol and acetonitrile with ammonium acetate was essential to achieve baseline resolution of these compounds. We investigated the effects of ammonium acetate concentration, temperature, applied voltage, and capillary length on separation efficiency. The nonaqueous capillary electrophoresis and laser‐based thermo‐optical absorbance detection technique was used to study the metabolism of amitriptyline by Cunninghamella elegans . Sample preparation procedures were simplified for fast screening of the parent drug and its metabolites. Reproducible electropherograms were obtained from replicate cultures of C. elegans growing in the presence of amitriptyline.