Premium
Separation of oligonucleotides and DNA fragments by capillary electrophoresis in dynamically and permanently coated capillaries, using a copolymer of acrylamide and β‐ D ‐glucopyranoside as a new low viscosity matrix with high sieving capacity
Author(s) -
Chiari Marcella,
Damin Francesco,
Melis Alessandra,
Consonni Roberto
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191817
Subject(s) - copolymer , capillary electrophoresis , acrylamide , monomer , polymer , oligonucleotide , capillary action , viscosity , electrophoresis , polyacrylamide , matrix (chemical analysis) , chemistry , polymerization , chromatography , chemical engineering , buffer (optical fiber) , polymer chemistry , materials science , dna , organic chemistry , biochemistry , composite material , engineering , telecommunications , computer science
New copolymers of acrylamide and β‐ D ‐glucopyranoside were synthesized and characterized. The different reactivity of the two monomers towards radical polymerization meant we could control the growth of the polymer chains whose length was inversely related to the number of glucose residues incorporated in the copolymers. The properties of these polymers were investigated in the separation of oligonucleotides and double‐stranded DNA by capillary electrophoresis (CE) in coated and uncoated capillaries. The new copolymers were a suitable matrix for CE due to their high‐resolving capacity and low viscosity. We also looked into the advantages of a new method of dynamic suppression of electroosmotic flow based on the addition of small amounts (0.03–0.05%) of dimethylacrylamide to the sieving and to the running buffer. A complete test was run on the reproducibility and efficiency of separations carried out in a permanently and dynamically coated capillary, and the advantages and disadvantages of the two methods were compared.