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Capillary electrophoretic separation of 1 to 10 kbp sized dsDNA using poly(ethylene oxide) solutions in the presence of electroosmotic counterflow
Author(s) -
Chen HsuanShen,
Chang HuanTsung
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191816
Subject(s) - ethylene oxide , capillary electrophoresis , polymer , electrophoresis , capillary action , chemistry , polyacrylamide , chromatography , electro osmosis , analytical chemistry (journal) , oxide , poly ethylene , materials science , ethylene , polymer chemistry , copolymer , organic chemistry , composite material , catalysis
DNA fragments of 1 to 10 kbp in length were separated by capillary electrophoresis (CE), using poly(ethylene oxide) (PEO) solutions in the presence of electroosmotic flow. The technique requires filling the capillary with the polymer solution by means of electroosmotic flow (EOF). Separation times of 6–7 min in PEO solutions ranging from 0.3 to 8 × 10 6 M r at 375 V/cm were sufficient to separate the 11 components of the dsDNA ladder (0.5 to 10 kbp) by size. The migration behavior of the double‐stranded (ds)DNA fragments, interpreted by “Ferguson plot analysis”, in the system is indistinguishable from that previously reported for capillary zone electrophoresis (CZE) in a polyacrylamide solution without EOF. Potential advantages of conducting CZE using polymer solutions in the presence of EOF are: (i) Possibility of long migration times on short columns; (ii) possibility of introducing relatively viscous, high M r polymer solutions into narrow capillaries; (iii) possibility of establishing polymer concentration gradients in capillaries; (iv) possibility of concentrating the starting zone by balancing electrophoretic migration and electroosmotic transport.