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Finding a universal low viscosity polymer for DNA separation (II)
Author(s) -
Heller Christoph
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191813
Subject(s) - polymer , capillary electrophoresis , matrix (chemical analysis) , oligonucleotide , viscosity , dna , electrophoresis , macromolecule , chromatography , chemistry , separation method , gel electrophoresis , materials science , organic chemistry , biochemistry , composite material
When investigating the use of different polymers for capillary electrophoresis we found that poly‐ N,N ‐dimethylacrylamide (pDMA) has a very low viscosity compared to other polymers of comparable molecular mass and resolving power. This makes it a potentially useful matrix for DNA separation in multi‐capillary electrophoresis, where short cycle times or low pressure for matrix replacement are preferred. We have characterized this matrix by systematic studies on concentration, chain length and field strength dependence. It is shown that pDMA performs well for the separation of oligonucleotides and double‐stranded DNA fragments. Together with the application of DNA sequencing, pDMA is a universal polymer for the separation of biological macromolecules.