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Identification of animal species in ground meat mixtures by multivariate analysis of isoelectric focusing protein profiles
Author(s) -
Skarpeid HansJacob,
Kvaal Knut,
Hildrum Kjell Ivar
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191811
Subject(s) - isoelectric focusing , multivariate statistics , isoelectric point , chromatography , multivariate analysis , chemistry , analytical chemistry (journal) , composition (language) , biological system , mathematics , biology , statistics , biochemistry , enzyme , linguistics , philosophy
The present work investigates the possibility of constructing a multivariate calibration model to predict the composition of ground meat with respect to animal species, based on intensity profiles from isoelectric focusing of water‐soluble proteins in mixtures of ground meat. Samples containing various amounts of beef, pork and turkey meat were analyzed by isoelectric focusing in immobilized pH‐gradients. The gels were photographed and the images transferred to a digital format. The digital images were processed in order to reduce background and optimize signal strength. Resulting gel profiles were analyzed by multivariate regression allowing the determination of sample composition with prediction errors close to 10%.