DNA preparation and efficient microsatellite analysis from insect hemolymph | Zendy

Gerken Thomas | Zendy; Kurtz Joachim | Zendy; Sauer Klaus P. | Zendy; Lubjuhn Thomas | Zendy

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DNA preparation and efficient microsatellite analysis from insect hemolymph

Author(s) -

Gerken Thomas,

Kurtz Joachim,

Sauer Klaus P.,

Lubjuhn Thomas

Publication year - 1998

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150191804

Subject(s) - hemolymph , microsatellite , insect , mating , biology , dna , phylogenetic tree , population , computational biology , genetics , evolutionary biology , zoology , biological system , chemistry , biochemistry , ecology , gene , medicine , allele , environmental health

A simple and time‐saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1–5 μL of insect hemolymph in a Chelex 100‐suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.

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