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DNA preparation and efficient microsatellite analysis from insect hemolymph
Author(s) -
Gerken Thomas,
Kurtz Joachim,
Sauer Klaus P.,
Lubjuhn Thomas
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191804
Subject(s) - hemolymph , microsatellite , insect , mating , biology , dna , phylogenetic tree , population , computational biology , genetics , evolutionary biology , zoology , biological system , chemistry , biochemistry , ecology , gene , medicine , allele , environmental health
A simple and time‐saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1–5 μL of insect hemolymph in a Chelex 100‐suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.