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Analysis of isoquinoline alkaloids in medicinal plants by capillary electrophoresis—mass spectrometry
Author(s) -
Sturm Sonja,
Stuppner Hermann
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191639
Subject(s) - chemistry , isoquinoline , sanguinarine , chromatography , mass spectrometry , capillary electrophoresis , papaveraceae , ranunculaceae , electrospray ionization , analytical chemistry (journal) , mass spectrum , alkaloid , stereochemistry , botany , biology
The technique of capillary electrophoresis – mass spectrometry (CE‐MS) was applied for determination of isoquinoline alkaloids in crude methanolic extracts of medicinal plants. For the CE separations ammonium formate buffer solutions (70 or 100 m M , pH 3.0 or 4.0) containing 10% methanol or 20–60% acetonitrile as additives were used. The applied voltage was 25 kV, the thermostating temperature was kept constant at 25°C. Coupling with the mass spectrometer was performed via an atmospherical pressure ionization (API) interface and the electrospray ionization technique (ESI). As sheath liquid 5 m M formic acid in acetonitrile at a flow rate of 3 μL/min was used. The spray voltage was 4.5 kV and the temperature of the heated capillary was chosen to be 200°C. Detection in the positive ionization mode resulted in mass spectra showing either the molecular ions [M] + or the protonated molecular ions [M+H] + . The presented method allows detection and identification of isoquinoline alkaloids in crude methanolic extracts of medicinal plants as Eschscholzia californica C HAM . (Papaveraceae), Hydrastis canadensis L. (Ranunculaceae), Berberis vulgaris L. (Berberidaceae), Jateorhiza palmata (L AM .) M IERS (Menispermaceae) and Chelidonium majus L. (Papaveraceae).