Premium
Separation of etoposide phosphate and methotrexate by capillary zone electrophoresis using UV detection with a high sensitivity cell
Author(s) -
Mrestani Yahya,
Neubert Reinhard
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191638
Subject(s) - capillary electrophoresis , chromatography , etoposide , chemistry , phosphate , electrophoresis , biochemistry , biology , chemotherapy , genetics
Etoposide phosphate and methotrexate are important anti‐tumor chemotherapeutic agents. Our previously presented capillary zone electrophoresis (CZE) method using a high sensitivity cell (Z‐cell) for quantitative analysis in biological media (urine, plasma) showed good precision and accuracy. The present results show that the investigation using a capillary with high sensitivity cell led to an approximately 10‐fold improvement of the detection limit compared to standard capillaries. Plasma and urine samples were analyzed by using a calibration curve for drug concentrations between 0.1 and 100 μg/mL. Good detection limits and good relative standard deviations of the migration times and of the peak areas were observed in these experiments.