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Determination of atropine in biological fluids by micellar electrokinetic capillary chromatography in the presence of strychnine and tetracaine
Author(s) -
Plaut Olivier,
Staub Christian
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191634
Subject(s) - micellar electrokinetic chromatography , chromatography , chemistry , detection limit , tetracaine , atropine , strychnine , capillary electrophoresis , capillary action , sodium , anesthesia , lidocaine , biochemistry , medicine , organic chemistry , materials science , composite material
The identification and quantitation of atropine, in whole blood and gastric contents in the presence of strychnine and tetracaine is described. This method uses liquid‐liquid extraction and micellar electrokinetic chromatography (MECC). Separations are made using a 50 cm long capillary and a borate/phosphate buffer at pH 9.2 with 50 m M sodium dodecyl sulfate (SDS). Linearity was established for the three compounds between 1.0 and 100 μ/mL, using scopolamine as internal standard. The limit of detection for atropine was estimated at 0.06 μ/mL and the limit of quantitation at 0.2 μg/mL. The run time is less than 30 min. Alternate parameters are proposed to reduce the run time to under 10 min. The method was applied to a forensic post‐mortem case.