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Determination of bupivacaine and three of its metabolites in rat urine by capillary electrophoresis
Author(s) -
Schieferecke Mark A.,
McLaughlin Kieran J.,
Faibushevich Alexander A.,
Lunte Craig E.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191633
Subject(s) - bupivacaine , urine , chromatography , chemistry , capillary electrophoresis , extraction (chemistry) , local anesthetic , prilocaine , pharmacology , anesthesia , medicine , biochemistry
A capillary electrophoretic (CE) method for the analysis of urinary extracts of the local anesthetic, bupivacaine, and its three main metabolites, desbutylbupivacaine, 3′‐hydroxybupivacaine, and 4′‐hydroxybupivacaine, in rat urine has been developed. The limits of detection were 0.22 μ M for desbutylbupivacaine and bupivacaine, 0.15 μ M for 3′‐hydroxybupivacaine, and 0.16 μ M for 4′‐hydroxybupivacaine. The linear range was from 0.7 μ M to 16.8 μ M for all four compounds. Migration time and peak height reproducibilities, and extraction efficiencies were determined for all four compounds. Peak height reproducibilities ( n = 5) for the overall method were improved through the use of prilocaine as an internal standard. Peak height reproducibilities were 5.6% RSD for desbutylbupivacaine and bupivacaine, and 9.9% RSD for 3′‐hydroxybupivacaine and 4′‐hydroxybupivacaine. Migration time reproducibilities ( n = 5) were 2.4% for all compounds. Urine samples were collected from rats administered therapeutic doses of bupivacaine and extracted using a solid‐phase extraction method (SPE). Separation of bupivacaine and its metabolites was achieved in 15 min.