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Analyte identification in capillary electrophoretic separation techniques
Author(s) -
Kok Steven J.,
Velthorst Nel H.,
Gooijer Cees,
Th. Brinkman Udo A.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191604
Subject(s) - analyte , capillary electrophoresis , capillary electrochromatography , mass spectrometry , analytical chemistry (journal) , chromatography , chemistry , electrochromatography , ultraviolet , capillary action , materials science , optoelectronics , composite material
Abstract A review on applications of on‐line hyphenation in capillary electrophoresis and capillary electrochromatography for the identification of migrating analytes is presented. There is an urgent need for unambiguous analyte identification by combining spectral information and observed migration times, because the parameters influencing the migration times and separation efficiencies in these separation techniques are not easily controlled, especially when real samples containing unknown interferences have to be analyzed. The spectrometric techniques covered here are ultraviolet and visible radiation (UV/Vis) absorption, fluorescence including fluorescence line‐narrowing spectroscopy, Raman spectroscopy, nuclear magnetic resonance and mass spectrometry. Attention is essentially confined to literature reports in which the extra information provided by the detector is really used for identification purposes, especially in real‐life samples, while the interfacing as such and analyte detectabilities in standard solutions are only briefly discussed. This article covers an extensive fraction of the literature published on this topic until the beginning of 1998.