Premium
Separation of 1‐phenyl‐3‐methyl‐5‐pyrazolone derivatives of monosaccharides by capillary electrochromatography
Author(s) -
Suzuki Shigeo,
Yamamoto Mayumi,
Kuwahara Yuri,
Makiura Kyoko,
Honda Susumu
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191518
Subject(s) - monosaccharide , chemistry , chromatography , elution , capillary electrochromatography , high performance liquid chromatography , column chromatography , acetonitrile , glucuronic acid , polysaccharide , organic chemistry , capillary electrophoresis
1‐Phenyl‐3‐methyl‐5‐pyrazolone (PMP) derivatives of component monosaccharides in glycoproteins (fucose, galactose, mannose, N ‐acetylgalactosamine and N ‐acetylglucosamine) and epimeric aldopentoses (arabinose, lyxose, ribose and xylose) were well separated from each other by capillary electrochromatography on a Hypersil ODS column with a mixture of 50 m M N ‐(2‐hydroxyethyl)piperazine‐2′‐(2‐ethanesulfonic acid) buffer, pH 6.0 to ∼ 6.3, and acetonitrile (2.2:1 v/v) as eluent. The elution of these compounds showed relatively strong dependence on the pH and concentration of the buffer salts contained in the eluent, as compared to the elution by pressure‐driven high‐performance liquid chromatography (HPLC) on the same stationary phase, but separation of PMP‐monosaccharides was better than that by HPLC. Retention times of PMP‐monosaccharides were highly reproducible with a relative standard deviation (RSD) of ∼ 0.6%, and quantification with an RSD less than 5% could be achieved using 3‐O‐methylglucose as an internal standard.